Fluorescein PAGE Analysis of Microsatellite-Primed PCR: A Fast and Efficient Approach for Genomic Fingerprinting
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چکیده
منابع مشابه
Fluorescein PAGE analysis of microsatellite-primed PCR: a fast and efficient approach for genomic fingerprinting.
partial digests are difficult to control and are not feasible when the number of internal sites is high. A second solution would be to amplify the desired region with PCR using a primer set that flanks the terminal EcoRI and HindIII sites. In this case, a unique restriction site would be incorporated into the downstream primer in such a way that HindIII digestion would not be required for the c...
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probe. Retention of the probe on the dialysis bag using the electroelution protocol and inefficient binding of the probe to glass beads (as seen with ds DNA below 0.5 kb in size) contribute to probe losses. Since the ss probe does not need to be purified from the LMP agarose gel, loss of the probe associated with the two established protocols described here (particularly with the glass bead pro...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00286bm03